COLOR CODES EMB1 & tadpole EMBRYOS
Codes taken from MRC listings, Oct 1981 Nov. 6, 2009; translated by DHH
These two middle stage wild type embryos were reconstructed in serial thin sections by John Sulston and colleagues in the 1970s and 1980s, particularly for the anatomy of the embryonic nose. Available thin sections were photographed at ~3,000X and the EM negatives copied onto large format prints.
For “tadpole”, almost all cells of the bodywall were colored using a violet color code (#1-220+). Many cells of the pharynx were marked with a green code, but none of those cells were identified.
Below we show an extensive all blue color code for EMB1 prints #152-800. In EMB1 prints #1-151, prominent sensory dendrites, glial structures, and nerve ring cells were reconstructed using at least four different colors (red, green, brown and blue) (download the Color Code for tadpole to see that list).
Of special note, the four embryonic CEM neurons are still found in both animals, prior to their programmed cell death in a later stage of hermaphrodite development. And the XXX cell bodies still lie in the anterior nose.
See Sulston, J.E., Schierenberg, E., White, J.G. and Thomson, J.N. (1983) Dev. Biol. 100: 64-119. “The embryonic cell lineage of the nematode C. elegans”
Codes for Codes for
1 ASKL
2 ASIL
3 ADLL
4 ASJL
5 ASEL
6 ASGL
7 AWBL
8 AWCL
9 AWAL
10 ASHL
11 AFDL
12 ADFL
13 AUAL
14 ASKR
15 ADLR
16 ASIR
17 AWCR
18 AWBR
19 ASGR
20 ASHR
21 AWAR
22 ASER
23 ASJR
24 AUAR
25 ADFR
26 AFDR
27 RIAR
28 AVAR
29 AVER
30 SIBVR
31 AIBR
32 RIBR
33 RIMR
34 AVBR
35 RICR
36 AIZR
37 AINR
38 RIVR
39 AVJR
40 AVHR
41 AVDR
42 DEsh R
43 FLPR
44 ADER
45
46 amphid sh R
47 SAAVR
48 RMDR
49 SMDVR
50 RMDVR
51 OLQVR 111
52 BAGR 118
53 GLRR
54 AINL
55 AVHL
56 AVDL
57 AVJL
58 RIVL
59 amphid sh L
60 RIAL
61 AVAL
62 SAAVL 75
63 AVEL
64 RMDL
65 SIBVL
66 AIBL
67 DEsh L
68 ADEL
69 RIBL
70 RIML
71 AVBL
72 AIZL
73 RICL
74 GLRL 107
75 SMDVL
76 RMDVL
77 BAGL 72 / br20
78 OLQVL 77
79 OLLL 67 / br19
80 URBL 38
81 URYVL 65
82 IL2L 28
83 IL1L 48 / br21
84 RIPL 55
85 RMEL 49
86 OLso L 68
87 CEPso DL 98
88 ILsh L 39 / br17
89 OLsh L 27 / br18
90 OLQso VL 25
91 1st
hyp ring 37
92 OLQsh VL 34
93 amphid so L 12 / br11
94 post
arcade VL 7 / 14
95 ILso L 17 / br16
96 OLLR 97
97 URBR 70
98 RIPR 79
99 RMER 80
100 OLso R 60
101 IL2R 82 / br7
102 IL1R 81 / br8
103 CEPso DR 61
104 ILsh R 78 / br6
105 OLQsh VR 53
106 amphid so R 32 / br9
107 1st
hyp ring?
108 OLsh R 44
109 ILso R 40 / br5
110 post
arcade VR 13 / 15
111 post
arcade DR 24 / 8
112 URXL 100
113 mesoblast
114 mesoblast 104
115 URYR 103
116 GLRDR
117 GLRDL 90
118 CEM
DR 102
119 CEM
DL 95
120 CEPDR 87
121 CEPDL 69
122 ALA 92
123 OLQDL 91 / br3
124 OLQDR 105 / br1
125 CEPsh DR 86
126 RID 104
127 URYDR 93 / br2
128 URYDL 74 / br4
129 CEPsh DL 71
130 GLRVL 120
131 GLRVR 139
132 SAADL 122
133 RIR 132
134 SAADR
135 CEPVL 116
136 RIH 155/115
137 SIBDR
138 CEPVR 136
139 SIBDL 141
140 SMBDR
141 E
duct cell 179
142 RMDDR
143 RMDDL 134
144 SMBDL 158
145 SMDDR
146 E
duct cell
147 AVL
148 SMBVR
149 SMBVL
150 SMDDL
151 SIADR
152 SIADL
153 AIAR
154 AIAL
155 AIYR
156 blast
cell
157 SIAVR
158 blast
cell
159 SIAVL
160 RIS
161 AIMR
162 AIYL
163 RIGR
164 AVKR
165 AIML
166 RIGL
167 RMEV 110
168 CEPsh VL 89
169 IL1VR 84 / 3
170 IL1VL 85
171 CEPsh VR 123
172 CEM
VR 119
173 CEM
VL 109
174 IL2VR 76 / 4
175 IL2VL 62 / br3
176 ILsh VR 56 / 2
177 ILsh VL 41 / br2
178 CEPso VL 63
179 URAVL 42
180 URAVR 83
181 1st
hyp ring 30
182 CEPso VR 88
183 ILso VL 26
/ br1
184 ILso VR 35 / 11
185 URYVR 114
186 2nd
hyp ring 16
187 post
arcade V 11 / 16
188 ant
arcade V 9 / 1
189 OLQso VR 54
190 26
191 ILsh DR 43
192 OLso DR 31
193 OLQso DL 21
194 IL2DL 36
195 OLQsh DL 29
196 ILsh DL 33
197 IL1DL 47
198 4th
hyp ring (3 cells) 2 / 7
199 URADL 50
200 URADR 73
201 RMED 66
202 IL1DR 64
203 OLsh DR 46
204 FLPL
205
206
207
208
209
210
211
212
213
214
215 gland
cell
216 AVKR
217
218
219
220
221
222
223 gland
cell
224
225
226
227
228
229
230
231
232
233